WGS of isolate 58 - SRA

SRX23394557: WGS of isolate 58
1 ILLUMINA (Illumina MiSeq) run: 156,500 spots, 85M bases, 42.1Mb downloads

Design: Bacterial cells centrifuged, pellet resuspended with buffer, RNaseA and lysozyme, then lysis buffer and proteinase K added, extraction was done by heating. Charge Switch gDNA mini bacteria kit was used to recover DNA and used Nextera XT DNA library preparation kit. Nanodrop and Qubit was used for quality control and quantification. Illumina Miseq Desktop Sequencer using Miseq Reagent kit v3 for 600 cycles.

Submitted by: Iowa State University

Study: NGS of Disease-associated Streptococcus suis isolates

PRJNA1069106 • SRP485963 • All experiments • All runs

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Streptococcus suis, a pathogenic bacterium, poses a significant threat to pigs, leading to pneumonia, arthritis, polyserositis, endocarditis, and meningitis. While naturally occurring in the normal microbiota of pigs, this pathogen is frequently identified in the nasal, tonsil, and stool samples of swine. Our study focused on sequencing isolates linked to neurologic disease within the USA swine herd. This investigation aims to enhance our understanding of the diversity and the presence or absence of virulence-associated genes in Streptococcus suis isolates associated with neurologic disease in pigs.

Sample:

SAMN39611911 • SRS20255474 • All experiments • All runs

Organism: Aerococcus urinaeequi

Library:

Name: Disease Associated 58

Instrument: Illumina MiSeq

Strategy: WGS

Source: GENOMIC

Selection: unspecified

Layout: PAIRED

Runs: 1 run, 156,500 spots, 85M bases, 42.1Mb

Run	# of Spots	# of Bases	Size	Published
SRR27728770	156,500	85M	42.1Mb	2024-09-25

ID:: 31615526

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